The Cheating Karta Hai Tu meme originates from the 2011 Indian film Singham. In this Bollywood movie, Jaikant Shikre (the character played by Prakash Raj) says this dialogue to Inspector Bajirao Singham (the character played by Ajay Devgn) when the inspector beings the entire Goa Police Force to fight the soon-to-be Minister of Goa Government. The popular use of Cheating Karta Hai Tu meme is to poke fun at someone who is throwing tantrums after losing.
